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If there is a sufficiently acidic environment between 15 and 3 on the pH scale the vagus nerve along with hormone secretions stimulate the release of pepsinogen into the stomach. The digestive power of pepsin is greatest at the acidity of normal gastric juice pH 1525. The Ph Stability And Ph Activity Of Pepsin 3 Download Scientific Diagram Pepsin is produced by stomach cells. . As such it works best in acidic conditions 1 8 9. Pepsin works really well in your stomach but its inactive in other parts of your body. Each and every enzyme works best in a particular range of conditions that reflect the environment in which it evolved. The enzyme named pepsin is released by some chief cells that can be found in the stomach. Enzymes exposed to environments outside their preferred temperature or acidity -- also called pH -- range dont function. It does not actually form. In acid reflux stomach contents make their w...